Antifungal Activity and Action Mode of Cuminic Acid from the Seeds of Cuminum cyminum L. against Fusarium oxysporum f. sp. Niveum (FON) Causing Fusarium Wilt on Watermelon.
نویسندگان
چکیده
In order to develop a novel biofungicide, the antifungal activity and action mode of cuminic acid from the seed of Cuminum cyminum L. against Fusarium oxysporum f. sp. niveum (FON) on watermelon was determined systematically. In this study, the median effective concentration (EC50) value for cuminic acid in inhibiting mycelial growth of FON was 22.53 μg/mL. After treatment with cuminic acid, the mycelial morphology was seriously influenced; cell membrane permeability and glycerol content were increased markedly, but pigment and mycotoxin (mainly fusaric acid) were significantly decreased. Synthesis genes of bikaverin (Bike1, Bike2 and Bike3) and fusaric acid (FUB1, FUB2, FUB3 and FUB4) both were downregulated compared with the control, as confirmed by quantitative RT-PCR. In greenhouse experiments, cuminic acid at all concentrations displayed significant bioactivities against FON. Importantly, significant enhancement of activities of SOD, POD, CAT and decrease of MDA content were observed after in vivo cuminic acid treatment on watermelon leaves. These indicated that cuminic acid not only showed high antifungal activity, but also could enhance the self-defense system of the host plant. Above all, cuminic acid showed the potential as a biofungicide to control FON.
منابع مشابه
Antifungal Activity and Action Mode of Cuminic acid
Watermelon fusarium wilt caused by Fusarium oxysporum f.sp. niveum (FON) is a 13 destructive soil-borne disease throughout the world leading to serious economic losses and limit 14 watermelon production. Cuminic acid, extracted from the seed of Cuminum cyminum L., belongs to 15 benzoic acid analogues. In this study, the median effective concentration (EC50) values for cuminic 16 acid in inhibit...
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ورودعنوان ژورنال:
- Molecules
دوره 22 12 شماره
صفحات -
تاریخ انتشار 2017